We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
RAP2.6L and jasmonic acid-responsive genes are expressed upon Arabidopsis hypocotyl grafting but are not needed for cell proliferation related to healing.
- Authors
Matsuoka, Keita; Yanagi, Raiki; Yumoto, Emi; Yokota, Takao; Yamane, Hisakazu; Satoh, Shinobu; Asahina, Masashi
- Abstract
Key message: Jasmonic acid and RAP2.6L are induced upon wounding but are not involved in cell proliferation during healing in Arabidopsis hypocotyls.Abstract: Plants produce jasmonic acid in response to wounding, but its role in healing, if any, has not been determined. Previously, the jasmonic acid-induced transcription factor, RAP2.6L, related to APETALA 2.6-like, was identified as a spatially expressed factor involved in tissue reunion in partially incised flowering stems of <italic>Arabidopsis</italic>. In the present study, we investigated the function of JA and RAP2.6L on wound healing using an Arabidopsis hypocotyl-grafting system, in which separated tissues are reattached by vascular tissue cell proliferation. The jasmonic acid-responsive genes <italic>AOS</italic> and <italic>JAZ10</italic> were transiently expressed immediately after grafting. We confirmed that the endogenous content of jasmonic acid-Ile, which is the bioactive form of jasmonic acid, increased in hypocotyls 1 h after grafting. Morphological analysis of the grafted tissue revealed that vascular tissue cell proliferation occurred in a similar manner in wild-type <italic>Arabidopsis</italic>, the jasmonic acid-deficient mutant <italic>aos</italic>, the jasmonic acid-insensitive mutant <italic>coi1</italic>, and in <italic>Arabidopsis</italic> that had been exogenously treated with jasmonic acid. <italic>RAP2.6L</italic> expression was also induced during graft healing. Because <italic>RAP2.6L</italic> expression occurred during graft healing in <italic>aos</italic> and <italic>coi1</italic>, its expression must be regulated via a jasmonic acid-independent pathway. The <italic>rap2.6L</italic> mutant and dominant repressor transformants for RAP2.6L showed normal cell proliferation during graft healing. Taken together, our results suggest that JA and RAP2.6L, induced by grafting, are not necessary for cell proliferation process in healing.
- Subjects
JASMONIC acid; GENE expression; ARABIDOPSIS; CELL proliferation; WOUND healing
- Publication
Plant Molecular Biology, 2018, Vol 96, Issue 6, p531
- ISSN
0167-4412
- Publication type
Article
- DOI
10.1007/s11103-018-0702-4