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- Title
Overexpression of thioredoxin reductase 1 inhibits migration of HEK-293 cells.
- Authors
Jolanta Sroka; Agata Antosik; Jarosław Czyż; Ivan Nalvarte; Jerker M. Olsson; Giannis Spyrou; Zbigniew Madeja
- Abstract
Background information. TrxR (thioredoxin reductase), in addition to protecting against oxidative stress, plays a role in the redox regulation of intracellular signalling pathways controlling, among others, cell proliferation and apoptosis. The aim of the present study was to determine whether TrxR1 is involved in the regulation of cell migration.Results. Stably transfected HEK-293 (human embryonic kidney) cells which overexpress cytosolic TrxR1 (HEK-TrxR15 and HEK-TrxR11 cells) were used in the present study. We found that the stimulation of cell motility induced by PKC (protein kinase C) activators, PMA and DPhT (diphenyltin), was inhibited significantly in the HEK-TrxR15 and HEK-TrxR11 cells compared with control cells. The overexpression of TrxR1 also inhibited characteristic morphological changes and reorganization of the F-actin cytoskeleton induced by PMA and DPhT. In addition, the selective activation of PKCδ by DPhT was inhibited in cells that overexpressed cytosolic TrxR1. Furthermore, rottlerin, a selective inhibitor of PKCδ, and PKCδ siRNA (small interfering RNA), suppressed the morphological changes induced by DPhT in the control cells.Conclusions. The overexpression of TrxR1 inhibits migration of HEK-293 cells stimulated with PMA and DPhT. Moreover, our observations suggest that this effect is mediated by the inhibition of PKCδ activation.
- Subjects
HUMAN embryos; CELL migration; GENE expression; OXIDATION-reduction reaction
- Publication
Biology of the Cell (Wiley-Blackwell), 2007, Vol 099, Issue 12, p677
- ISSN
0248-4900
- Publication type
Article
- DOI
10.1042/bc20070024