We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
HOXD8 hypermethylation as a fully sensitive and specific biomarker for biliary tract cancer detectable in tissue and bile samples.
- Authors
Loi, Eleonora; Zavattari, Cesare; Tommasi, Alessandro; Moi, Loredana; Canale, Matteo; Po, Agnese; Sabato, Claudia; Vega-Benedetti, Ana Florencia; Ziranu, Pina; Puzzoni, Marco; Lai, Eleonora; Faloppi, Luca; Rullán, María; Carrascosa, Juan; Amat, Irene; Urman, Jesús M; Arechederra, Maria; Berasain, Carmen; Ferretti, Elisabetta; Casadei-Gardini, Andrea
- Abstract
<bold>Background: </bold>Biliary tract cancers (BTC) are rare but highly aggressive tumours with poor prognosis, usually detected at advanced stages. Herein, we aimed at identifying BTC-specific DNA methylation alterations.<bold>Methods: </bold>Study design included statistical power and sample size estimation. A genome-wide methylation study of an explorative cohort (50 BTC and ten matched non-tumoral tissue samples) has been performed. BTC-specific altered CpG islands were validated in over 180 samples (174 BTCs and 13 non-tumoral controls). The final biomarkers, selected by a machine-learning approach, were validated in independent tissue (18 BTCs, 14 matched non-tumoral samples) and bile (24 BTCs, five non-tumoral samples) replication series, using droplet digital PCR.<bold>Results: </bold>We identified and successfully validated BTC-specific DNA methylation alterations in over 200 BTC samples. The two-biomarker panel, selected by an in-house algorithm, showed an AUC > 0.97. The best-performing biomarker (chr2:176993479-176995557), associated with HOXD8, a pivotal gene in cancer-related pathways, achieved 100% sensitivity and specificity in a new series of tissue and bile samples.<bold>Conclusions: </bold>We identified a novel fully efficient BTC biomarker, associated with HOXD8 gene, detectable both in tissue and bile by a standardised assay ready-to-use in clinical trials also including samples from non-invasive matrices.
- Subjects
PROTEINS; BILE duct tumors; GENETIC mutation; DNA methylation; BILE; RESEARCH funding; TRANSCRIPTION factors
- Publication
British Journal of Cancer, 2022, Vol 126, Issue 11, p1783
- ISSN
0007-0920
- Publication type
journal article
- DOI
10.1038/s41416-022-01738-1