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- Title
Preparation and Epitope Mapping of a Monoclonal Antibody against Tn a Bd 27K, a Major Wheat Allergen.
- Authors
Komiyama, Nobuko; Hiemori, Miki; Kimoto, Masumi; Suzuki, Makiko; Yamashita, Hiromi; Takahashi, Yoshitaka; Tsuji, Hideaki
- Abstract
The article presents a study on the epitope mapping and development of the monoclonal antibody (mAb) against a major wheat allergen, Tri a Bd 27K. It cites the use of several eight week old female mice that undergo immunization and the spleen cells were fused with P3NS1 myelome cells to identify the wheat allergen. Moreover, it notes that the mAb identifies the allergen from wheat flour and the epitope on the allergen to the mAb was found to be amino acid sequence of Tri a Bd 27K.
- Subjects
MONOCLONAL antibodies; ALLERGENS; WHEAT genetics; EPITOPES; LABORATORY mice; AMINO acid sequence; HYPOALLERGENIC products
- Publication
Bioscience, Biotechnology & Biochemistry, 2009, Vol 73, Issue 9, p2113
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1271/bbb.90224