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- Title
Purification and Characterization of a-Glucosidase I from Japanese Honeybee (Apis cerana japonica) and Molecular Cloning of Its cDNA.
- Authors
Wongchawalit, Jintanart; Yamamoto, Takeshi; Nakai, Hiroyuki; Young-Min Kim; Sato, Natsuko; Nishimoto, Mamoru; Okuyama, Masayuki; Mori, Haruhide; Saji, Osamu; Chanpen Chanchao; Wongsiri, Siriwat; Surarit, Rudee; Svasti, Jisnuson; Chiba, Seiya; Kimura, Atsuo
- Abstract
The article discusses the purification and characterization of a-Glucosidase (JHGase I) from a Japanese subspecies of eastern honeybee (Apis cerena japonica). The JHGase I was isolated from component 1 to undergo further purification using CM-Tojopearl 650M and Sepharel S-100. JHGase I showed unusual kinetic features: the negative cooperative behavior on the intrinsic reaction and positive cooperative on turanose. In addition, JHGase I is a member of a-amylase family enzymes.
- Subjects
ENZYMES; ENZYMOLOGY; HONEYBEES; BEHAVIOR; TURANOSE; FAMILIES
- Publication
Bioscience, Biotechnology & Biochemistry, 2006, Vol 70, Issue 12, p2889
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1271/bbb.60302