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- Title
Immuno-spin trapping detection of antioxidant/pro-oxidant properties of zinc or selenium on DNA and protein radical formation via hydrogen peroxide.
- Authors
Deletioglu, Vedia; Tuncay, Erkan; Toy, Aysegul; Atalay, Mustafa; Turan, Belma
- Abstract
Trace elements can participate in the catalysis of group-transfer reactions and can serve as their structural components. However, most of them including zinc and selenium have multifunctional roles in biological environments such as antioxidant and/or pro-oxidant effects, as concentration-dependent manner. Although it has been demonstrated the antioxidant actions of either selenium or zinc compounds, there are several documents pointing out their pro-oxidant/oxidant roles in biological systems. Here we have used ELISA-based immuno-spin trapping, a method for detection of free radical formation, to detect whether or not a zinc compound, Zn(PO), or a selenium compound, NaSeO, has antioxidant and/or pro-oxidant effect on 5,5-Dimethyl-1-Pyrroline-N-Oxide (DMPO)-DNA nitrone adducts induced with Cu(II)-HO-oxidizing system in in vitro preparations. Second, we examined whether this technique is capable to demonstrate the different DMPO-protein nitrone adduct productions in isolated protein crude of hearts from normal rats (CON) or rats with metabolic syndrome (MetS). Our data demonstrated that either Zn (100 µM) or $${\text{SeO}}_{3}^{ - 2}$$ (50 nM) has very strong antioxidant action against 200 µM HO-induced DMPO-DNA nitrone adduct production, whereas their higher concentrations have apparent pro-oxidant actions. We also used verification by Western blotting analysis whether immuno-spin trapping can be used to assess HO-induced DMPO-protein nitrone adducts in heart protein crudes. Our Western blot data further confirmed the ELISA-data from proteins and demonstrated how Zn or $${\text{SeO}}_{3}^{ - 2}$$ are dual-functioning ions such as antioxidant at lower concentrations while pro-oxidant at higher concentrations. Particularly, our present data with $${\text{SeO}}_{3}^{ - 2}$$ in DMPO-protein nitrone adducts, being in line with our previous observation on its dual-actions in ischemia/reperfusion-induced damaged heart, have shown that this ion has higher pro-oxidant actions over 50 nM in MetS-group compared to that of CON group.
- Subjects
ANTIOXIDANTS; THERAPEUTIC use of zinc; SELENIUM; HYDROGEN peroxide; REACTIVE oxygen species; LIPID peroxidation (Biology); GLUTATHIONE; THERAPEUTICS
- Publication
Molecular & Cellular Biochemistry, 2015, Vol 409, Issue 1/2, p23
- ISSN
0300-8177
- Publication type
Article
- DOI
10.1007/s11010-015-2508-x