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- Title
Effects of N -hexacosanol on nitric oxide synthase system in diabetic rat nephropathy.
- Authors
Shinichi Okada; Motoaki Saito; Emi Kazuyama; Takuya Hanada; Yasuo Kawaba; Atsushi Hayashi; Keisuke Satoh; Susumu Kanzaki
- Abstract
Abstract We attempted to clarify the effects of cyclohexenonic long-chain fatty alcohol (N-hexacosanol) on nitric oxide synthase (NOS) in streptozotocin-induced diabetic nephropathy. After induction of experimental diabetes with streptozotocin, rats were maintained for 8 weeks with or without treatment by N-hexacosanol (8 mg/kg i.p. every day). Urinary albumin excretion, blood chemistry, immunoblot analysis, and real-time polymerase chain reactions (real-time PCR) of endothelial nitric oxide synthase (eNOS), inducible NOS (iNOS), and neuronal NOS (nNOS) were investigated. Although N-hexacosanol had no effects on serum glucose or insulin level, it normalized serum creatinine and urinary albumin excretion. N-hexacosanol was found to improve the diabetes-induced alterations in the eNOS, iNOS, and nNOS protein and their mRNA levels. Histologically, N-hexacosanol inhibited the progression to glomerular sclerosis. Our data suggest that N-hexacosanol improves diabetes-induced NOS alterations in the kidney, resulting in the amelioration of diabetic nephropathy.
- Subjects
NITRIC oxide; KIDNEY diseases; LABORATORY rats; DIABETIC nephropathies
- Publication
Molecular & Cellular Biochemistry, 2008, Vol 315, Issue 1/2, p169
- ISSN
0300-8177
- Publication type
Article
- DOI
10.1007/s11010-008-9804-7