We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Stimulus-evoked outer segment changes in rod photoreceptors.
- Authors
Xiaohui Zhao; Damber Thapa; Benquan Wang; Yiming Lu; Shaoyan Gai; Xincheng Yao
- Abstract
Rod-dominated transient retinal phototropism (TRP) has been recently observed in freshly isolated mouse and frog retinas. Comparative confocal microscopy and optical coherence tomography revealed that the TRP was predominantly elicited from the rod outer segment (OS). However, the biophysical mechanism of rod OS dynamics is still unknown. Mouse and frog retinal slices, which displayed a cross-section of retinal photoreceptors and other functional layers, were used to test the effect of light stimulation on rod OSs. Time-lapse microscopy revealed stimulus-evoked conformational changes of rod OSs. In the center of the stimulated region, the length of the rod OS shrunk, while in the peripheral region, the rod OS swung toward the center region. Our experimental observation and theoretical analysis suggest that the TRP may reflect unbalanced rod disc-shape changes due to localized visible light stimulation.
- Subjects
PHOTORECEPTORS; PHOTOTAXIS; COHERENCE (Optics); CONFOCAL microscopy; SPECTRUM analysis
- Publication
Journal of Biomedical Optics, 2016, Vol 21, Issue 6, p065006-1
- ISSN
1083-3668
- Publication type
Article
- DOI
10.1117/1.JBO.21.6.065006