We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Rotational imaging optical coherence tomography for full-body mouse embryonic imaging.
- Authors
Chen Wu; Sudheendran, Narendran; Singh, Manmohan; Larina, Irina V.; Dickinson, Mary E.; Larin, Kirill V.
- Abstract
Optical coherence tomography (OCT) has been widely used to study mammalian embryonic development with the advantages of high spatial and temporal resolutions and without the need for any contrast enhancement probes. However, the limited imaging depth of traditional OCT might prohibit visualization of the full embryonic body. To overcome this limitation, we have developed a new methodology to enhance the imaging range of OCT in embryonic day (E) 9.5 and 10.5 mouse embryos using rotational imaging. Rotational imaging OCT (RI-OCT) enables full-body imaging of mouse embryos by performing multiangle imaging. A series of postprocessing procedures was performed on each cross-section image, resulting in the final composited image. The results demonstrate that RI-OCT is able to improve the visualization of internal mouse embryo structures as compared to conventional OCT.
- Subjects
OPTICAL coherence tomography; EMBRYOLOGY; EMBRYONIC physiology; MICE embryology; LABORATORY mice
- Publication
Journal of Biomedical Optics, 2016, Vol 21, Issue 2, p1
- ISSN
1083-3668
- Publication type
Article
- DOI
10.1117/1.JBO.21.2.026002