We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
In vivoearly diagnosis of gastric dysplasia using narrow-band image-guided Raman endoscopy.
- Authors
Zhiwei Huang; Mads Sylvest Bergholt; Wei Zheng; Kan Lin; Khek Yu Ho; Ming Teh; Khay Guan Yeoh
- Abstract
We first report on the implementation of a novel narrow-band image-guided Raman endoscopy technique for in vivodiagnosis of gastric dysplasia. High-quality in vivoRaman spectra can be acquired from normal and dysplastic gastric mucosal tissue within 0.5 secunder narrow-band image (NBI) guidance at gastroscopy. Significant differences are observed in in vivoRaman spectra between normal (n=54)and dysplastic (n=18)gastric tissue from 30 gastric patients, particularly in the spectral ranges of 825 to 950, 1000 to 1100, 1250 to 1500, and 1600 to 1800 cm−1, which primarily contain signals related to proteins, nucleic acids, and lipids. The multivariate analysis i.e., principal components analysis (PCA) and linear discriminant analysis (LDA), together with the leave-one tissue site-out, cross validation on in vivogastric Raman spectra yields a diagnostic sensitivity of 94.4 (1718)and specificity of 96.3 (5254)for distinction of gastric dysplastic tissue. This study suggests that narrowband image-guided Raman endoscopy associated with PCA-LDA diagnostic algorithms has potential for the noninvasive, in vivoearly diagnosis and detection of gastric precancer during clinical gastroscopic examination.
- Subjects
GASTRIC diseases; DYSPLASIA; GASTROSCOPY; MEDICAL imaging systems; RAMAN spectroscopy; PRINCIPAL components analysis; DIAGNOSIS; PATIENTS
- Publication
Journal of Biomedical Optics, 2010, Vol 15, Issue 3, p037017
- ISSN
1083-3668
- Publication type
Article
- DOI
10.1117/1.3420115