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- Title
Isolation and identification of <italic>Salmonella pullorum</italic> bacteriophage YSP2 and its use as a therapy for chicken diarrhea.
- Authors
Tie, Kunyuan; Yuan, Yuyu; Yan, Shiqing; Yu, Xi; Zhang, Qiuyang; Xu, Huihui; Zhang, Yang; Gu, Jingmin; Sun, Changjiang; Lei, Liancheng; Han, Wenyu; Feng, Xin
- Abstract
<italic>Salmonella pullorum</italic> is the major pathogen that is harmful to the poultry industry in developing countries, and the treatment of chicken diarrhea caused by <italic>S. pullorum</italic> has become increasingly difficult. In this study, a virulent bacteriophage YSP2, which was able to specifically infect <italic>Salmonella</italic>, was isolated and characterized. Phage YSP2 was classified in the Siphoviridae family and had a short latent period of 10 min. No bacterial virulence- or lysogenesis-related ORF is present in the YSP2 genome, making it eligible for use in phage therapy. Experiments in vivo investigated the potential use of phages as a therapy against diarrhea in chickens caused by <italic>S. pullorum</italic> in a chicken diarrhea model, demonstrating that a single oral administration of YSP2 (1 × 1010 PFU/mL, 80 μL/chicken) 2 h after <italic>S. pullorum</italic> oral administration at a double median lethal dose was sufficient to protect chickens against diarrhea. Gross inspection showed that YSP2 can effectively reduce organ damage and significantly relieve hemorrhage in the intestine and liver tissue. Moreover, YSP2 can maintain a high curative effect when diluted to 108 PFU/mL. In light of its therapeutic effect on chicken diarrhea, YSP2 may serve as an alternative treatment strategy for infections caused by <italic>S. pullorum.</italic>
- Subjects
SALMONELLA pullorum; BACTERIOPHAGES; CHICKEN diseases; DIARRHEA; SIPHOVIRIDAE
- Publication
Virus Genes, 2018, Vol 54, Issue 3, p446
- ISSN
0920-8569
- Publication type
Article
- DOI
10.1007/s11262-018-1549-0