We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
RNase I regulates Escherichia coli 2',3'-cyclic nucleotide monophosphate levels and biofilm formation.
- Authors
Fontaine, Benjamin M.; Martin, Kevin S.; Garcia-Rodriguez, Jennifer M.; Jung, Claire; Briggs, Laura; Southwell, Jessica E.; Xin Jia; Weinert, Emily E.
- Abstract
Regulation of nucleotide and nucleoside concentrations is critical for faithful DNA replication, transcription and translation in all organisms and has been linked to bacterial biofilm formation. Unusual 20,30-cyclic nucleotide monophosphates (20,30-cNMPs) recently were quantified in mammalian systems and previous reports have linked these nucleotides to cellular stress and damage in eukaryotes, suggesting an intriguing connection with nucleotide/nucleoside pools and/or cyclic nucleotide signaling. This work reports the first quantification of 20,30-cNMPs in Escherichia coli and demonstrates that 20,30-cNMP levels in E. coli are generated specifically from RNase I-catalyzed RNA degradation, presumably as part of a previously unidentified nucleotide salvage pathway. Furthermore, RNase I and 20,30-cNMP levels are demonstrated to play an important role in controlling biofilm formation. This work identifies a physiological role for cytoplasmic RNase I and constitutes the first progress toward elucidating the biological functions of bacterial 20,30-cNMPs.
- Subjects
RIBONUCLEASES; DNA replication; EUKARYOTES; NUCLEOTIDES; ESCHERICHIA coli
- Publication
Biochemical Journal, 2018, Vol 475, Issue 8, p1491
- ISSN
0264-6021
- Publication type
Article
- DOI
10.1042/BCJ20170906