We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Female-Specific DNA Sequences in the Chicken Genome.
- Authors
Granevitze, Zur; Blum, Shula; Cheng, Hans; Vignal, Alain; Morisson, Mireille; Ben-Ari, Giora; David, Lior; Feldman, Marcus William; Weigend, Steffen; Hillel, Jossi
- Abstract
Eight in silico W-specific sequences from the WASHUC1 chicken genome assembly gave female-specific PCR products using chicken DNA. Some of these fragments gave female-specific products with turkey and peacock DNA. Sequence analysis of these 8 fragments (3077 bp total) failed to detect any polymorphisms among 10 divergent chickens. In contrast, comparison of the DNA sequences of chicken with those of turkey and peacock revealed a nucleotide difference every 25 and 28 bp, respectively. Radiation hybrid mapping verified that these amplicons exist only on chromosome W. The homology of 6 W-specific fragments with chromo-helicase-DNA-binding gene and expressed sequenced tags from chicken and other species indicate that these fragments may have or have had a biological function. These fragments may be used for early sexing in commercial chicken and turkey flocks.
- Subjects
NUCLEOTIDE sequence; GENOMES; CHICKENS; GENETIC polymorphisms; GENE mapping; CHROMOSOMES
- Publication
Journal of Heredity, 2007, Vol 98, Issue 3, p238
- ISSN
0022-1503
- Publication type
Article
- DOI
10.1093/jhered/esm010