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- Title
P4HB: A novel diagnostic and prognostic biomarker for bladder carcinoma.
- Authors
YUCAI WU; YIJI PENG; BAO GUAN; ANBANG HE; KUNLIN YANG; SHIMING HE; YANQING GONG; XUESONG LI; LIQUN ZHOU
- Abstract
Prolyl 4-hydroxylase, beta polypeptide (P4HB) protein is an endoplasmic reticulum (ER) molecular chaperone protein and has been reported to be overexpressed in multiple tumor types. However, the role of P4HB in bladder cancer (BLCA) has not yet been elucidated. The aim of the present study was to investigate the prognostic value of P4HB and the association between clinicopathological characteristics and P4HB in BLCA. P4HB expression levels were assessed through The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases, and validated by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blot analysis in BLCA tissues and cells. A total of 69 pairs of tumor and normal samples were used to analyze the expression of P4HB via immunohistochemical staining. A co-expression network and functional enrichment analyses were conducted to investigate the biological function of P4HB in BLCA. The protein-protein interaction (PPI) network was constructed by Search Tool for the Retrieval of Interacting Genes. The results showed that P4HB was highly
- Subjects
PROGNOSIS; MOLECULAR chaperones; WESTERN immunoblotting; BIOMARKERS; IMMUNOSTAINING
- Publication
Oncology Letters, 2021, Vol 21, Issue 2, p1
- ISSN
1792-1074
- Publication type
Article
- DOI
10.3892/ol.2020.12356