We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Spectral properties and chromosphere rotation of phytochrome bound to substituted Sepharose.
- Authors
Sundqvist, Christer; Ekelund, Nils G. A.
- Abstract
Brushite purified phytochrome from Avena sativa L. cv. Sol II was bound to phenyl Sepharose, octyl Sepharose, CNBr-activated Sepharose and to anti-phytochrome immunoglobulins immobilized on Sepharose. The spectral properties of phytochrome bound to anti-phytochrome immunoglobulins and to phenyl Sepharose were similar to phytochrome in solution. Phytochrome bound to CNBr-activated Sepharose or to octyl Sepharose showed reduced Pfr formation after red irradiation. The reversal to P1 with far-red light was only partial but a further increase at 667 nm took place slowly in the dark. A peak at 657 nm was seen in the difference spectrum between CNBr-activated Sepharose-bound phytochrome kept in darkness and the identical sample immediately after a far-red irradiation. The change in linear dichroism at 660 nm and 730 nm, induced by plane polarized red or far-red light, was measured. It was computed that the long-wavelength transition moment of phytochrorne had an average rotation angle of 31.5° or 180°-31.5°. The substrate used for immobilization had a limited effect on the rotation angle. Phytochrome immobilized on CNBr-activated Sepharose gave an angle of 27.8° and phytochrome immobilized on phenyl Sepharose gave an angle of 32.6°.
- Subjects
PHYTOCHROMES; IMMUNOGLOBULINS; SEPHAROSE; IRRADIATION; DICHROISM; PLANT pigments
- Publication
Physiologia Plantarum, 1986, Vol 66, Issue 2, p185
- ISSN
0031-9317
- Publication type
Article
- DOI
10.1111/j.1399-3054.1986.tb02406.x