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- Title
Diagnosing genetic disease. Successful metaphase chromosome analysis of human elongated spermatids using mouse oocytes.
- Authors
Araki, Y.; Ogawa, S.; Ohno, M.; Yoshizawa, M.; Motoyama, M.; Araki, S.; Aslam, I.; Fishel, S.
- Abstract
Human elongated spermatids from azoospermic patients were inserted into mouse oocytes by intracytoplasmic sperm injection (ICSI). The injection resulted in survival rates of 46.5% (180 out of 387) and activation rates of 36.1% (65 out of 180). The rate of two pronuclear (2PN) formation was 35.4% (23 out of 65). Only 34.8% (eight out of 23) metaphase chromosome spreads from 2PN zygotes could be analysed; however, all were of normal karyotype. Cytogenetic analysis at the first metaphase revealed that human elongated spermatid chromosomes were able to undergo replication in a heterogeneous environment.
- Subjects
HUMAN chromosomes; SPERMATOZOA; MICE; SCIENTIFIC experimentation
- Publication
Molecular Human Reproduction, 1999, Vol 5, Issue 8
- ISSN
1360-9947
- Publication type
Article
- DOI
10.1093/molehr/5.8.784