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- Title
Enterocin TW21, a novel bacteriocin from dochi-isolated Enterococcus faecium D081821.
- Authors
Chang, S.‐Y.; Chen, Y.‐S.; Pan, S.‐F.; Lee, Y.‐S.; Chang, C.‐H.; Yu, B.; Wu, H.‐C.
- Abstract
Aims Purification and characterization of a novel bacteriocin produced by strain Enterococcus faecium D081821. Methods and Results Enterococcus faecium D081821, isolated from the traditional Taiwanese fermented food dochi (fermented black beans), was previously found to produce a bacteriocin against Listeria monocytogenes and some Gram-positive bacteria. This bacteriocin, termed enterocin TW21, was purified from culture supernatant by ammonium sulfate precipitation, Sep- Pak C18 cartridge, ion-exchange and gel filtration chromatography. Mass spectrometry analysis showed the mass of the peptide to be approximately 5300·6 Da. The N-terminal amino acid sequencing yielded a partial sequence NH2- ATYYGNGVYx NTQK by Edman degradation, and it contains the consensus class IIa bacteriocin motif YGNGV in the N-terminal region. The open reading frame ( ORF) encoding the bacteriocin was identified from the draft genome sequence of Enterococcus faecium D081821, and sequence analysis of this peptide indicated that enterocin TW21 is a novel bacteriocin. Conclusions Enterococcus faecium D081821 produced a bacteriocin named enterocin TW21, the molecular weight and amino acid sequence both revealed it to be a novel bacteriocin. Significant and Impact of Study A new member of class IIa bacteriocin was identified. This bacteriocin shows great inhibitory ability against L. monocytogenes and could be applied as a natural food preservative.
- Subjects
ENTEROCINS; BACTERIOCINS; ENTEROCOCCUS faecium; FERMENTED foods; LISTERIA monocytogenes; LACTIC acid bacteria
- Publication
Journal of Applied Microbiology, 2013, Vol 115, Issue 3, p673
- ISSN
1364-5072
- Publication type
Article
- DOI
10.1111/jam.12265