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- Title
A Human Monoclonal Antibody Reacting with Merkel Cells: Immunofluorescence, Immunoperoxidase, and Immunoelectron Microscopy.
- Authors
Saurat, J.-H.; Chavaz, P.; Carraux, P.; Didierjean, L.
- Abstract
A human monoclonal, mu, kappa, cold agglutinin antibody of the rare specificity Pr h (serum and proper eluates) was used in immunofluorescence and immunoperoxidase techniques and in immunoelectron microscopy on rabbit lip specimens. Pr h antibody strongly reacted with scattered cells in epidermis, which were demonstrated to be Merkel cells by electron microscopy; no nerve fibers were stained. In immunoelectron microscopy (IEM), a strong reaction was seen within the cytoplasm and around the granules. This is the first IEM staining of Merkel cells (MC) so far reported; it demonstrates the expression of a carbohydrate differentiation antigen in MC. The availability of a potent monoclonal antibody reacting with MC but not with neighboring epidermal cells in rabbit lip offers a new tool for the study of several aspects of MC biology, including antigenic properties and kinetics.
- Subjects
AGGLUTININS; MONOCLONAL antibodies; MERKEL cells; IMMUNOFLUORESCENCE; IMMUNOENZYME technique; ELECTRON microscopy
- Publication
Journal of Investigative Dermatology, 1983, Vol 81, Issue 3, p249
- ISSN
0022-202X
- Publication type
Article
- DOI
10.1111/1523-1747.ep12518261