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- Title
Selective ex vivo expansion of cytomegalovirus-specific CD4<sup>+</sup> and CD8<sup>+</sup> T lymphocytes using dendritic cells pulsed with a human leucocyte antigen A*0201-restricted peptide.
- Authors
Vannucchi, Alessandro M.; Glinz, Stephanie; Bosi, Alberto; Caporale, Roberto; Rossi-Ferrini, Pierluigi
- Abstract
Adoptive transfer of ex vivo-generated cytomegalovirus (CMV)-specific T lymphocytes may be effective in preventing CMV disease in allogeneic haematopoietic stem cell transplantation (HSCT) recipients. We developed a procedure for expansion of CMV-specific T lymphocytes based on the antigen-presenting function of donor dendritic cells (DCs), pulsed with a human leucocyte antigen A*0201-restricted pp65 nonamer peptide. CMV-specific T lymphocytes were identified following induction of interferon γ (IFN-γ) secretion prompted by peptide exposure. Both CD8+ and CD4+ CMV-specific T lymphocytes were selectively produced in these cultures and showed CMV-restricted cytotoxicity. The simultaneous and selective expansion of CD4+ and CD8+ CMV-specific lymphocytes might be instrumental for more efficient in vivo function of infused CMV-specific lymphocytes.
- Subjects
CYTOMEGALOVIRUSES; T cells; LEUCOCYTES
- Publication
British Journal of Haematology, 2001, Vol 113, Issue 2, p479
- ISSN
0007-1048
- Publication type
Article
- DOI
10.1046/j.1365-2141.2001.02777.x