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- Title
The Impact of Acute Nutritional Interventions on the Plasma Proteome.
- Authors
Vernardis, Spyros I.; Demichev, Vadim; Lemke, Oliver; Grüning, Nana-Maria; Messner, Christoph; White, Matt; Pietzner, Maik; Peluso, Alina; Collet, Tinh-Hai; Henning, Elana; Gille, Christoph; Campbell, Archie; Hayward, Caroline; Porteous, David J.; Marioni, Riccardo E.; Mülleder, Michael; Zelezniak, Aleksej; Wareham, Nicholas J.; Langenberg, Claudia; Farooqi, I. Sadaf
- Abstract
therefore important to deeply characterize the human nutritional responses. Objective: Endocrine parameters and the metabolome of human plasma are rapidly responding to acute nutritional interventions such as caloric restriction or a glucose challenge. It is less well understood whether the plasma proteome would be equally dynamic, and whether it could be a source of corresponding biomarkers. Methods: We used high-throughput mass spectrometry to determine changes in the plasma proteome of i) 10 healthy, young, male individuals in response to 2 days of acute caloric restriction followed by refeeding; ii) 200 individuals of the Ely epidemiological study before and after a glucose tolerance test at 4 time points (0, 30, 60, 120 minutes); and iii) 200 random individuals from the Generation Scotland study. We compared the proteomic changes detected with metabolome data and endocrine parameters. Results: Both caloric restriction and the glucose challenge substantially impacted the plasma proteome. Proteins responded across individuals or in an individual-specific manner. We identified nutrient-responsive plasma proteins that correlate with changes in the metabolome, as well as with endocrine parameters. In particular, our study highlights the role of apolipoprotein C1 (APOC1), a small, understudied apolipoprotein that was affected by caloric restriction and dominated the response to glucose consumption and differed in abundance between individuals with and without type 2 diabetes. Conclusion: Our study identifies APOC1 as a dominant nutritional responder in humans and highlights the interdependency of acute nutritional response proteins and the endocrine system.
- Subjects
PROTEOMICS; LOW-calorie diet; METABOLOMICS
- Publication
Journal of Clinical Endocrinology & Metabolism, 2023, Vol 108, Issue 8, p2087
- ISSN
0021-972X
- Publication type
Article
- DOI
10.1210/clinem/dgad031