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- Title
A study of transcriptome in leaf rust infected bread wheat involving seedling resistance gene Lr28.
- Authors
Sharma, Chanchal; Saripalli, Gautam; Kumar, Santosh; Gautam, Tinku; Kumar, Avneesh; Rani, Sushma; Jain, Neelu; Prasad, Pramod; Raghuvanshi, Saurabh; Jain, Mukesh; Sharma, J. B.; Prabhu, K. V.; Sharma, P. K.; Balyan, H. S.; Gupta, P. K.
- Abstract
Leaf rust disease causes severe yield losses in wheat throughout the world. During the present study, high-throughput RNA-Seq analysis was used to gain insights into the role of Lr28 gene in imparting seedling leaf rust resistance in wheat. Differential expression analysis was conducted using a pair of near-isogenic lines (NILs) (HD 2329 and HD 2329 + Lr28) at early (0 h before inoculation (hbi), 24 and 48 h after inoculation (hai)) and late stages (72, 96 and 168 hai) after inoculation with a virulent pathotype of pathogen Puccinia triticina. Expression of a large number of genes was found to be affected due to the presence/absence of Lr28. Gene ontology analysis of the differentially expressed transcripts suggested enrichment of transcripts involved in carbohydrate and amino acid metabolism, oxidative stress and hormone metabolism, in resistant and/or susceptible NILs. Genes encoding receptor like kinases (RLKs) (including ATP binding; serine threonine kinases) and other kinases were the most abundant class of genes, whose expression was affected. Genes involved in reactive oxygen species (ROS) homeostasis and several genes encoding transcription factors (TFs) (most abundant being WRKY TFs) were also identified along with some ncRNAs and histone variants. Quantitative real-time PCR was also used for validation of 39 representative selected genes. In the long term, the present study should prove useful in developing leaf rust resistant wheat cultivars through molecular breeding. Wheat is an important staple food crop worldwide, but leaf rust disease in this crop causes >50% yield losses. A transcriptomic approach using RNA-Seq in a pair of near-isogenic lines (NILs) resolved differential expression of hundreds of genes in presence/absence of Lr28. This will help build a better understanding of host–pathogen interaction (HPI) involving leaf rust in bread wheat.
- Subjects
TRANSCRIPTOMES; LEAF rust of wheat; SEEDLINGS; HORMONE metabolism; PUCCINIA triticina
- Publication
Functional Plant Biology, 2018, Vol 45, Issue 10, p1046
- ISSN
1445-4408
- Publication type
Article
- DOI
10.1071/FP17326