We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
An isoflavone cladrin prevents high-fat diet-induced bone loss and inhibits the expression of adipogenic gene regulators in 3T3-L1 adipocyte.
- Authors
Gautam, Jyoti; Khedgikar, Vikram; Choudhary, Dharmendra; Kushwaha, Priyanka; Dixit, Preeti; Singh, Divya; Maurya, Rakesh; Trivedi, Ritu
- Abstract
Objective This study evaluates the effect of isoflavone cladrin on high-fat diet (HFD)-induced bone loss and adipogenesis. Methods Thirty-two 4-week-old male C57BL/6J mice were divided into four groups: a standard diet group, a HFD group and HFD group with cladrin (5 and 10 mg/kg per day orally) for 12 weeks. The effect of cladrin on bone micro-architecture, bone marrow cell lineages and hyperlipidaemia were assessed. For assessing anti-adipogenic activity of cladrin, 3T3-L1 cells were used. Key findings Cladrin attenuated HFD-induced hyperlipidaemia and bone loss by preserving bone micro-architecture and strength. Effect of cladrin was found at the level of bone marrow progenitor cells. Gene expression profile of cladrin-treated mice bone showed upregulation of osteoblast and downregulation of adipogenic transcription factors and increased OPG/RANKL ratio. Cladrin inhibited cellular lipid accumulation through downregulation of transcription factors such as PPAR-γ and C/EBP-α and modulated the expression of major adipokines involved behind obesity stimulation without eliciting cell cytotoxicity in 3T3-L1 adipocytes. Conclusion We conclude that cladrin may improve obesity-induced bone loss and hyperlipidaemia in mice fed HFD and adipogenesis in 3T3-L1 cells by modifying adipokines and could offer clinical benefits as a supplement to treat obesity-induced disorders.
- Subjects
THERAPEUTIC use of isoflavones; HIGH-fat diet; ADIPOGENESIS; LABORATORY mice; HYPERLIPIDEMIA
- Publication
Journal of Pharmacy & Pharmacology, 2016, Vol 68, Issue 8, p1051
- ISSN
0022-3573
- Publication type
Article
- DOI
10.1111/jphp.12562