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- Title
Myrrh mediates haem oxygenase-1 expression to suppress the lipopolysaccharide-induced inflammatory response in RAW264.7 macrophages.
- Authors
Cheng, Yu-Wen; Cheah, Khoot-Peng; Lin, Che-Wei; Li, Joe-Sharg; Yu, Wen-Yu; Chang, Ming Long; Yeh, Geng-Chang; Chen, Sheng-Hsuan; Choy, Cheuk-Sing; Hu, Chien-Ming
- Abstract
Objectives To elucidate a novel anti-inflammatory mechanism of myrrh against lipopolysaccharide (LPS)-induced inflammation. Methods RAW264.7 macrophages were cultured in DMEM and then cells were treated with LPS or LPS plus a myrrh methanol extract (MME) for 24 h. The culture medium was collected for determination of nitric oxide (NO), prostaglandin (PG)E2, interleukin (IL)-1 β, and tumour necrosis factor (TNF)- α, and cells were harvested by lysis buffer for Western blot analysis. Key findings Our data showed that treatment with the MME (1∼100 µg/ml) did not cause cytotoxicity or activate haem oxygenase-1 (HO-1) protein synthesis in RAW264.7 macrophages. Furthermore, the MME inhibited LPS-stimulated NO, PGE2, IL-1 β and TNF- α release and inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 protein expression. Zn(II) protoporphyrin IX, a specific inhibitor of HO-1, blocked the inhibition of iNOS and COX-2 expression by the MME. Conclusions These results suggest that among mechanisms of the anti-inflammatory response, the MME inhibited the production of NO, PGE2, IL-1 β and TNF- α by downregulating iNOS and COX-2 gene expression in macrophages and worked through the action of HO-1.
- Subjects
LIPOPOLYSACCHARIDES; INFLAMMATION; MACROPHAGES; NITRIC oxide; INTERLEUKINS
- Publication
Journal of Pharmacy & Pharmacology, 2011, Vol 63, Issue 9, p1211
- ISSN
0022-3573
- Publication type
Article
- DOI
10.1111/j.2042-7158.2011.01329.x