We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Occurrence and molecular analysis of extended-spectrum β-lactamase-producing <it>Proteus mirabilis</it> in Hong Kong, 1999–2002.
- Authors
P. L. Ho; Alex Y. M. Ho; K. H. Chow; River C. W. Wong; R. S. Duan; W. L. Ho; Gannon C. Mak; Kenneth W. Tsang; W. C. Yam; K. Y. Yuen
- Abstract
Objectives: A study was conducted to evaluate the occurrence and characterization of extended-spectrum β-lactamases (ESBLs) among blood isolates of Proteus mirabilis collected over a 4 year period in Hong Kong.Methods: Production of ESBLs among 99 consecutive and non-duplicate isolates was evaluated by the double-disc synergy test. The ESBLs were characterized by isoelectric focusing and PCR sequencing using specific primers. The epidemiological relationship of the isolates was studied by the Dienes test and PFGE.Results: ESBLs were identified in 13 isolates, from none in 1999–2000 and up to 18.5% (5/27) in 2001 and 25.8% (8/31) in 2002. The ESBL-producing isolates were more resistant to ceftriaxone than to ceftazidime, and were more likely than non-ESBL-producers to have resistance to ciprofloxacin (76.9% versus 14%) and gentamicin (38.5% versus 9.3%). The ESBL content included CTX-M-13 (n=8), CTX-M-14 (n=3), SHV-5 (n=2), TEM-11 (n=1), and an unidentified ESBL with a pI of 7.5. The Dienes test revealed that the genetic background in the 99 isolates was highly heterogeneous, with 54 distinct types among 92 isolates and seven were non-typeable. Among the 13 ESBL-producing isolates, five different backgrounds, including one cluster (Dienes-pulsotype A) with nine isolates, were identified by both Dienes test and PFGE, thus suggesting both clonal and multi-clonal spread of the CTX-M enzymes.Conclusions: Our findings indicate the emergence of CTX-M enzymes among P. mirabilis in Hong Kong. More ESBL screening of this species is required to improve their recognition.
- Publication
Journal of Antimicrobial Chemotherapy (JAC), 2005, Vol 55, Issue 6, p840
- ISSN
0305-7453
- Publication type
Article
- DOI
10.1093/jac/dki135