We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Up-regulation of mir-10b predicate advanced clinicopathological features and liver metastasis in colorectal cancer.
- Authors
Jiang, Hong; Liu, Jijun; Chen, Yingtao; Ma, Chong; Li, Baosong; Hao, Tao
- Abstract
Given the emerging role of micro RNA in tumor disease progression, we investigated the association between mi RNA 10b expression, liver metastasis, and clinicopathological of colorectal cancer ( CRC). Two hundred and forty-six pairs of samples (including CRC samples and normal adjacent tissues) from CRC patients were collected from May 2004 to May 2009. All samples verified to contain at least 80% tumor cells, and were immediately frozen in liquid nitrogen and stored at −80°C or fixed in 10% formalin for paraffin embedding. The expression of mi RNA-10b in CRC tissues was evaluated using a quantitative real-time polymerase chain reaction RT- PCR. Correlation between miR-10b expression and poor clinicopathological of CRC patients were analyzed using Student's t-tests and Chi-square tests. A Kaplan-Meier survival curve was generated following a log-rank test. miR-10b expression was up-regulated in CRC tissues (P < 0.0001) and in patients diagnosed as colorectal liver metastasis ( CLM) at initial involvement or during follow-up. When the Tumor Node Metastasis ( TNM) stage was taken into consideration, the expression levels of miR-10b were positively correlated with advanced TNM stages. In addition, the miR-10b expression of patients diagnosed as CLM at initial involvement was significantly higher than those without liver metastasis ( nCLM). Similarly, those patients developed with CLM during follow-up ( FCLM) was also markedly higher than those with nCLM. miR-10b expression was also found correlated with advanced stage ( P < 0.0001), lymph node metastasis ( P = 0.025), venous infiltration ( P = 0.007), poorer differentiation ( P = 0.002), and served as an independent prognostic factor of poor overall survival ( P < 0.0001). This study demonstrated the expression of miR-10b had strong potential to serve as a noninvasive biomarker for CRC prognosis and predicting liver metastasis.
- Subjects
CANCER invasiveness; MICRORNA; LIVER metastasis; COLON cancer; CANCER cells
- Publication
Cancer Medicine, 2016, Vol 5, Issue 10, p2932
- ISSN
2045-7634
- Publication type
Article
- DOI
10.1002/cam4.789