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- Title
Clinicopathological significance of RCAN2 production in gastric carcinoma.
- Authors
Yui Hattori; Kazuhiro Sentani; Shunsuke Shinmei; Htoo Zarni Oo; Takuya Hattori; Takeharu Imai; Yohei Sekino; Naoya Sakamoto; Naohide Oue; Hiroaki Niitsu; Takao Hinoi; Hideki Ohdan; Wataru Yasui
- Abstract
Aims: Gastric cancer (GC) is one of the leading causes of cancer-related death worldwide. Genes expressed only in cancer tissue may be useful biomarkers for cancer diagnosis and therapeutics. The aims of the present study were to analyse regulator of calcineurin 2 (RCAN2) in a large number of GCs, and to investigate how these expression patterns correlate with clinicopathological parameters and various markers. Methods and results: An immunohistochemical analysis of RCAN2 in 207 GC tissue samples showed that 110 (53%) GCs were positive for RCAN2. RCAN2-positive GCs were more advanced in terms of TNM classification and tumour stage than RCAN2-negative GCs. Furthermore, RCAN2 was an independent prognostic classifier for GC patients. The cell growth and invasiveness of RCAN2 small interfering RNA (siRNA)-transfected GC cell lines were less than those of the negative control siRNA-transfected cell lines, whereas those of RCAN2-transfected cells were significantly increased as compared with those of empty vector-transfected cells. RCAN2 siRNA inhibits the phosphorylation of AKT and p44/p42 (ERK1/2). RCAN2 was colocalised with EGFR, nuclear b-catenin, MMP7, laminin-c2, VEGF-A, and VEGF-C. Conclusion: These results suggest that RCAN2 is involved in tumour progression and is an independent prognostic classifier in patients with GC.
- Subjects
GASTRIC diseases; STOMACH cancer; CANCER diagnosis; CALCINEURIN; IMMUNOHISTOCHEMISTRY; CELL lines
- Publication
Histopathology, 2019, Vol 74, Issue 3, p430
- ISSN
0309-0167
- Publication type
Article
- DOI
10.1111/his.13764