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- Title
p16<sup>INK4A</sup> overexpression is frequently detected in tumour-free tonsil tissue without association with HPV.
- Authors
Klingenberg, Boris; Hafkamp, Harriët C; Haesevoets, Annick; Manni, Johannes J.; Slootweg, Pieter J.; Weissenborn, Soenke J.; Klussmann, Jens P.; Speel, Ernst-Jan M.
- Abstract
Klingenberg B, Hafkamp H C, Haesevoets A, Manni J J, Slootweg P J, Weissenborn S J, Klussmann J P & Speel E-J M (2010) Histopathology 56, 957–967 p16 INK4A overexpression is frequently detected in tumour-free tonsil tissue without association with HPV Aims: Oncogenic human papillomavirus (HPV) type 16 has been strongly associated with tonsillar squamous cell carcinoma (TSCC) and appears to be of prognostic significance. Because HPV+ TSCC also accumulates p16INK4A, this cyclin-dependent kinase inhibitor has been proposed as a potential biomarker for HPV in clinical diagnosis. The aim of this study was to determine the prevalence of HPV in tumour-free tonsillar tissue and the value of p16INK4A overexpression in predicting its presence. Methods and results: p16INK4A overexpression was detected by immunohistochemistry in tissue sections of tumour-free tonsils of 262 patients. They were treated for non-oncological reasons (snoring or chronic/recurrent tonsillitis) consisting of tonsillectomy. Genomic DNA isolated from these tissues was subjected to HPV-specific polymerase chain reaction (PCR) analysis. p16INK4A immunoreactivity was detected in 28% of samples in both crypt epithelium (49/177) and lymphoid germinal centres (52/187), which correlated with each other ( P < 0.0001). No reactivity was observed in superficial squamous cell epithelium. HPV16 and 18 were detected by PCR analysis in 2/195 cases (1%), which, however, were negative on fluorescence in situ hybridization analysis and discrepant on p16INK4A immunostaining. Conclusions: No proof was found for the presence of HPV in tumour-free tonsil tissue, despite increased p16INK4A expression in a quarter of tonsil cases. Other mechanisms than HPV infection are therefore implicated in p16INK4A up-regulation.
- Subjects
PAPILLOMAVIRUSES; POLYMERASE chain reaction; TONSILS; LYMPHOID tissue; IMMUNOHISTOCHEMISTRY; TONSILLECTOMY
- Publication
Histopathology, 2010, Vol 56, Issue 7, p957
- ISSN
0309-0167
- Publication type
Article
- DOI
10.1111/j.1365-2559.2010.03576.x