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- Title
Circulating IgA and IgE autoantibodies in antilaminin-332 mucous membrane pemphigoid.
- Authors
Natsuga, K.; Nishie, W.; Shinkuma, S.; Moriuchi, R.; Shibata, M.; Nishimura, M.; Hashimoto, T.; Shimizu, H.
- Abstract
Background Antilaminin-332 mucous membrane pemphigoid (MMP) is a chronic autoimmune bullous disease that is often associated with internal malignancy. IgG autoantibodies against laminin-332 in patients with MMP are well documented; however, IgA and IgE autoantibodies against laminin-332 have not yet been described. Objectives To characterize IgA and IgE autoantibodies binding to laminin-332 in sera from patients with antilaminin-332 MMP. Methods Sera and skin samples from four patients who met the following criteria were used: (i) subepidermal blistering lesions present on the mucous membranes; (ii) in vivo deposition of IgG along the epidermal basement membrane zone of sampled skin; (iii) circulating IgG antibasement membrane zone antibodies that react with the dermal side of salt-split normal human skin; and (iv) circulating IgG autoantibodies that do not show positivity against type VII collagen or 200-kDa protein (p200 antigen) in immunoblot analysis using dermal extracts. Circulating IgG/IgA/IgE class autoantibodies against laminin-332 were determined by immunoblotting. Results Circulating IgG autoantibodies against the γ2, α3/γ2, α3 and α3/β3/γ2 subunits of laminin-332 were demonstrated in sera from four patients, respectively. Serum from one of the four patients showed IgA reactivity with the α3/β3/γ2 subunits of laminin-332. Serum from one of the four patients showed IgE reactivity with the γ2 subunit of laminin-332. The control sera failed to display IgG/IgA/IgE reactivity to laminin-332. Conclusions In addition to IgG autoantibodies, circulating IgA and IgE autoantibodies against laminin-332 are detectable in a subset of patients with antilaminin-332 MMP.
- Subjects
AUTOANTIBODIES; AUTOIMMUNE diseases; AUTOIMMUNITY; IMMUNOGLOBULINS; IMMUNOBLOTTING
- Publication
British Journal of Dermatology, 2010, Vol 162, Issue 3, p513
- ISSN
0007-0963
- Publication type
Article
- DOI
10.1111/j.1365-2133.2009.09508.x