We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
The Burkholderia pseudomallei intracellular 'TRANSITome'.
- Authors
Heacock-Kang, Yun; McMillan, Ian A.; Norris, Michael H.; Sun, Zhenxin; Zarzycki-Siek, Jan; Bluhm, Andrew P.; Cabanas, Darlene; Norton, Robert E.; Ketheesan, Natkunam; Miller, Jeff F.; Schweizer, Herbert P.; Hoang, Tung T.
- Abstract
Prokaryotic cell transcriptomics has been limited to mixed or sub-population dynamics and individual cells within heterogeneous populations, which has hampered further understanding of spatiotemporal and stage-specific processes of prokaryotic cells within complex environments. Here we develop a 'TRANSITomic' approach to profile transcriptomes of single Burkholderia pseudomallei cells as they transit through host cell infection at defined stages, yielding pathophysiological insights. We find that B. pseudomallei transits through host cells during infection in three observable stages: vacuole entry; cytoplasmic escape and replication; and membrane protrusion, promoting cell-to-cell spread. The B. pseudomallei 'TRANSITome' reveals dynamic gene-expression flux during transit in host cells and identifies genes that are required for pathogenesis. We find several hypothetical proteins and assign them to virulence mechanisms, including attachment, cytoskeletal modulation, and autophagy evasion. The B. pseudomallei 'TRANSITome' provides prokaryotic single-cell transcriptomics information enabling high-resolution understanding of host-pathogen interactions. Prokaryotic cell transcriptomics has been limited to mixed or sub-population dynamics and individual cells within heterogeneous populations. Here the authors develop a 'TRANSITomic' approach to profile transcriptomes of single Burkholderia pseudomallei cells as they transit through host cell infection.
- Subjects
BURKHOLDERIA pseudomallei; SPATIOTEMPORAL processes; GENES; MELIOIDOSIS; TRANSCRIPTOMES; AUTOPHAGY
- Publication
Nature Communications, 2021, Vol 12, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-021-22169-1