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- Title
Plasmodium bergheiookinetes bind toAnopheles gambiaeandDrosophila melanogasterannexins.
- Authors
Kotsyfakis, Michalis; Ehret-Sabatier, Laurence; Siden-Kiamos, Inga; Mendoza, Jaqueline; Sinden, Robert E.; Louis, Christos
- Abstract
Using a proteomic approach we identified polypeptides fromAnopheles gambiaeandDrosophila melanogasterprotein extracts that selectively bind purifiedPlasmodium bergheiookinetesin vitro; these were two and three distinct polypeptides, respectively, with an apparent molecular weight of about 36 kDa. Combining two-dimensional electrophoresis and MALDI-TOF (matrix-associated laser desorption ionization time of flight) mass spectrometry we determined that the polypeptides correspond to isomorphs of the annexin B11 protein of the fruit fly. When protein extracts derived fromA. gambiaeandD. melanogastertissue culture cells were further fractionated, the binding activity matching the annexin protein could be localized in the fraction derived from cell membranes in both diptera. Antibody staining showed that annexin also binds to ookinetes during the invasion of the mosquito midgut. Finally, inclusion of antiannexin antisera in a mosquito blood meal impaired parasite development, suggesting a facilitating role for annexins in the infection of the mosquito byPlasmodium.
- Subjects
PLASMODIUM; MALARIA; ANOPHELES gambiae; DROSOPHILA melanogaster; ANNEXINS; CALCIUM-binding proteins; MICROBIAL peptides
- Publication
Molecular Microbiology, 2005, Vol 57, Issue 1, p171
- ISSN
0950-382X
- Publication type
Article
- DOI
10.1111/j.1365-2958.2005.04664.x