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- Title
Comparison of Multiple Displacement Amplification (MDA) and Multiple Annealing and Looping-Based Amplification Cycles (MALBAC) in Single-Cell Sequencing.
- Authors
Chen, Minfeng; Song, Pengfei; Zou, Dan; Hu, Xuesong; Zhao, Shancen; Gao, Shengjie; Ling, Fei
- Abstract
Single-cell sequencing promotes our understanding of the heterogeneity of cellular populations, including the haplotypes and genomic variability among different generation of cells. Whole-genome amplification is crucial to generate sufficient DNA fragments for single-cell sequencing projects. Using sequencing data from single sperms, we quantitatively compare two prevailing amplification methods that extensively applied in single-cell sequencing, multiple displacement amplification (MDA) and multiple annealing and looping-based amplification cycles (MALBAC). Our results show that MALBAC, as a combination of modified MDA and tweaked PCR, has a higher level of uniformity, specificity and reproducibility.
- Subjects
HAPLOTYPES; GENOMICS; SPERMATOZOA; DATA analysis; BIOTECHNOLOGY
- Publication
PLoS ONE, 2014, Vol 9, Issue 12, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0114520