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- Title
Nanomechanical In Situ Monitoring of Proteolysis of Peptide by Cathepsin B.
- Authors
Taeyun Kwon; Jinsung Park; Jaemoon Yang; Dae Sung Yoon; Sungsoo Na; Chang-Wan Kim; Jin-Suck Suh; Yong-Min Huh; Seungjoo Haam; Kilho Eom
- Abstract
Characterization and control of proteolysis of peptides by specific cellular protease is a priori requisite for effective drug discovery. Here, we report the nanomechanical, in situ monitoring of proteolysis of peptide chain attributed to protease (Cathepsin B) by using a resonant nanomechanical microcantilever immersed in a liquid. Specifically, the detection is based on measurement of resonant frequency shift arising from proteolysis of peptides (leading to decrease of cantilever's overall mass, and consequently, increases in the resonance). It is shown that resonant microcantilever enables the quantification of proteolysis efficacy with respect to protease concentration. Remarkably, the nanomechanical, in situ monitoring of proteolysis allows us to gain insight into the kinetics of proteolysis of peptides, which is well depicted by Langmuir kinetic model. This implies that nanomechanical biosensor enables the characterization of specific cellular protease such as its kinetics.
- Subjects
PROTEOLYSIS; PEPTIDES; PROTEINS; NANOELECTROMECHANICAL systems; PROTEIN metabolism; PROTEOLYTIC enzymes; ORGANIC compounds; AMINO acids; BIOSYNTHESIS
- Publication
PLoS ONE, 2009, Vol 4, Issue 7, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0006248