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- Title
p16<sup>INK4a</sup> Translation Suppressed by miR-24.
- Authors
Lal, Ashish; Hyeon Ho Kim; Abdelmohsen, Kotb; Kuwano, Yuki; Pullmann Jr., Rudolf; Srikantan, Subramanya; Subrahmanyam, Ramesh; Martindale, Jennifer L.; Xiaoling Yang; Ahmed, Fariyal; Navarro, Francisco; Dykxhoorn, Derek; Lieberman, Judy; Gorospe, Myriam
- Abstract
Background: Expression of the tumor suppressor p16INK4a increases during aging and replicative senescence. Methodology/Principal Findings: Here, we report that the microRNA miR-24 suppresses p16 expression in human diploid fibroblasts and cervical carcinoma cells. Increased p16 expression with replicative senescence was associated with decreased levels of miR-24, a microRNA that was predicted to associate with the p16 mRNA coding and 3'-untranslated regions. Ectopic miR-24 overexpression reduced p16 protein but not p16 mRNA levels. Conversely, introduction of antisense (AS)-miR-24 blocked miR-24 expression and markedly enhanced p16 protein levels, p16 translation, and the production of EGFP-p16 reporter bearing the miR-24 target recognition sites. Conclusions/Significance: Together, our results suggest that miR-24 represses the initiation and elongation phases of p16 translation.
- Subjects
SUPPRESSOR cells; TUMORS; AGING; FIBROBLASTS; CANCER cells; CERVICAL cancer; MESSENGER RNA; HOMEOSTASIS; GENE transfection; CELL culture
- Publication
PLoS ONE, 2008, Vol 3, Issue 3, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0001864