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- Title
Biochemical Analysis of Genome Functions Using Locus-Specific Chromatin Immunoprecipitation Technologies.
- Authors
Toshitsugu Fujita; Hodaka Fujii
- Abstract
To isolate specific genomic regions that retain their molecular interactions, allowing direct identification of chromatin-bound molecules, we developed two locus-specific chromatin immunoprecipitation (locus-specific ChIP) technologies, insertional ChIP (iChIP) and engineered DNA-binding molecule-mediated ChIP (enChIP) using the clustered regularly interspaced short palindromic repeats (CRISPR) system or transcription activator-like (TAL) proteins. Essentially, a locus-specific ChIP consists of locus-tagging and affinity purification and can be combined with downstream analyses to identify molecules associated with the target genomic regions. In this review, we discuss the applications of locus-specific ChIP to analyze the genome functions, including transcription and epigenetic regulation.
- Subjects
FUNCTIONAL genomics; CHROMATIN; CRISPRS; DNA-binding proteins; EPIGENETICS
- Publication
Gene Regulation & Systems Biology, 2016, Issue 10, p1
- ISSN
1177-6250
- Publication type
Article
- DOI
10.4137/GRSB.S32520