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- Title
Differential expression of human papillomavirus 16-, 18-, 52-, and 58-derived transcripts in cervical intraepithelial neoplasia.
- Authors
Baba, Satoshi; Taguchi, Ayumi; Kawata, Akira; Hara, Konan; Eguchi, Satoko; Mori, Mayuyo; Adachi, Katsuyuki; Mori, Seiichiro; Iwata, Takashi; Mitsuhashi, Akira; Maeda, Daichi; Komatsu, Atsushi; Nagamatsu, Takeshi; Oda, Katsutoshi; Kukimoto, Iwao; Osuga, Yutaka; Fujii, Tomoyuki; Kawana, Kei
- Abstract
Background: Human papillomavirus (HPV) infection is a primary cause of cervical cancer. Although epidemiologic study revealed that carcinogenic risk differs according to HPV genotypes, the expression patterns of HPV-derived transcripts and their dependence on HPV genotypes have not yet been fully elucidated. Methods: In this study, 382 patients with abnormal cervical cytology were enrolled to assess the associations between HPV-derived transcripts and cervical intraepithelial neoplasia (CIN) grades and/or HPV genotypes. Specifically, four HPV-derived transcripts, namely, oncogenes E6 and E6*, E1^E4, and viral capsid protein L1 in four major HPV genotypes—HPV 16, 18, 52, and 58—were investigated. Results: The detection rate of E6/E6* increased with CIN progression, whereas there was no significant change in the detection rate of E1^E4 or L1 among CIN grades. In addition, we found that L1 gene expression was HPV type-dependent. Almost all HPV 52-positive specimens, approximately 50% of HPV 58-positive specimens, around 33% of HPV 16-positive specimens, and only one HPV18-positive specimen expressed L1. Conclusions: We demonstrated that HPV-derived transcripts are HPV genotype-dependent. Especially, expression patterns of L1 gene expression might reflect HPV genotype-dependent patterns of carcinogenesis.
- Subjects
PAPILLOMAVIRUSES; PAPILLOMAVIRUS diseases; CERVICAL intraepithelial neoplasia; VIRAL proteins; GENE expression
- Publication
Virology Journal, 2020, Vol 17, Issue 1, p1
- ISSN
1743-422X
- Publication type
Article
- DOI
10.1186/s12985-020-01306-0