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- Title
Whole-genome sequencing approaches for Genomic characterization of high-level ciprofloxacin-resistant Campylobacter jejuni isolates from diarrheal patients.
- Authors
Eunkyung Shin; Jungsun Park; Joo Hyun Han; Hyun Ju Jeong; Jaeil Yoo; Junyoung Kim
- Abstract
배경 Campylobacter jejuni is one of the major causative pathogens of diarrheal diseases worldwide. In recent years, global attention has risen to the increase of emerging antimicrobial resistant C. jejuni. It has been known that the number of C. jejuni isolates displayed high antimicrobial resistance such as ciprofloxacin(CIP) in Korea. National surveillance studies are necessary to prevent and control the infection of multi-drug resistant strains 방법 From 2011 to 2020, total of 1,120 C. jejuni strains are isolated from the patients with diarrhea in Korea. Among them, 198 high-level ciprofloxacin resistant strains were selected (MICs; CIP >=16mg/L) for whole genome sequencing(WGS) using Illumina MiSeq platform. The analyses of genome characteristics were performed in Bacterial Analysis Pipeline version 1.0.4. and whole-genome single nucleotide polymorphism(wgSNP). The results were visualized by iTOL 결과 The analysis of draft genomes for these isolates indicated that their genome sizes were estimated between 1.60 and 1.86 Mb. The mean coverage depth varied from 14 to 289 times, and the overall GC content was about 30.5%. The maximum-likelihood phylogeny analysis from 57,060 wgSNPs show profiles of acquired antimicrobial resistance, virulence- and Guillain-Barre syndrome-associated genes 결론 Our data suggest that the WGS analysis is a powerful tool to identify genome characteristics and anti- microbial resistance genotype of pathogenic bacteria. The introduction of WGS for the prevention and management of antimicrobial-resistant bacteria can save time and cost more than before and enable ac- curate analysis.
- Subjects
SOUTH Korea; CAMPYLOBACTER jejuni; NUCLEOTIDE sequencing; WHOLE genome sequencing; GENOME size; SINGLE nucleotide polymorphisms; PATHOGENIC bacteria
- Publication
Infection & Chemotherapy, 2022, Vol 54, pS270
- ISSN
2093-2340
- Publication type
Article