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- Title
l-Ribulose production from l-arabinose by an l-arabinose isomerase mutant from Geobacillus thermodenitrificans.
- Authors
Yeom, Soo-Jin; Ji, Jung-Hwan; Yoon, Ran-Young; Oh, Deok-Kun
- Abstract
Geobacillus thermodenitrificans, with a double-site mutation in l-arabinose isomerase, produced 95 g l-ribulose l− 1 from 500 g l-arabinose l−1 under optimum conditions of pH 8, 70°C, and 10 units enzyme ml−1 with a conversion yield of 19% over 2 h. The half-lives of the mutated enzyme at 70 and 75°C were 35 and 4.5 h, respectively.
- Subjects
ENZYMES; GENETIC mutation; ISOMERASES; HYDROGEN-ion concentration; PROTEINS; BACILLUS (Bacteria)
- Publication
Biotechnology Letters, 2008, Vol 30, Issue 10, p1789
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-008-9746-x