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- Title
Cyclin E Amplification and Overexpression in Clear Cell Adenocarcinoma of the Ovary.
- Authors
Tsuda, Hiroshi; Bandera, Christina A.; Birrer, Michael J.; Hashiguchi, Yasunori; Berkowitz, Ross S.; Mok, Samuel C.
- Abstract
Objective: The purpose of this study is to compare DNA, mRNA and protein levels of the cyclin E between clear cell (CC) and serous (SC) ovarian carcinomas, and evaluate the relationship between cyclin E and p53 status. Method: We examined the DNA, mRNA and protein levels of cyclin E and the protein level of p53 in 44 CCs and 39 SCs using microdissected tissues. Results: Relative cyclin E mRNA expression was significantly higher in CC (3.62, 95% CI, 2.24–4.99) than in SC (1.75, 95% CI, 1.05–2.45; p = 0.0098). The percentage of positive nuclear staining of cyclin E was significantly higher in CC (48.3, 95% CI, 40.4–56.1) than SC (25.3, 95% CI, 17.4–33.3; p = 0.0001). The mRNA and protein expression of cyclin E was significantly correlated (r = 0.66, p < 0.0001). However, the correlation between relative DNA copy number and relative mRNA expression was not significant (r = –0.063; p = 0.66). Percentage of positive nuclear staining of cyclin E was significantly higher in p53 positive cases (51.8, 95% CI, 40.0–63.5) than p53 negative cases (36.2, 95% CI, 28.2–44.2; p = 0.028). Conclusions: Cyclin E expression is significantly higher in CC than in SC. Cyclin E expression is significantly related with p53 positivity. Copyright © 2004 S. Karger AG, Basel
- Subjects
CYCLINS; GENE expression; ADENOCARCINOMA; OVARIES; CANCER
- Publication
Oncology, 2004, Vol 67, Issue 3/4, p291
- ISSN
0030-2414
- Publication type
Article
- DOI
10.1159/000081330