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- Title
Human Adenovirus and Influenza A Virus Exacerbate SARS-CoV-2 Infection in Animal Models.
- Authors
Svyatchenko, Victor A.; Ternovoi, Vladimir A.; Lutkovskiy, Roman Y.; Protopopova, Elena V.; Gudymo, Andrei S.; Danilchenko, Nataliya V.; Susloparov, Ivan M.; Kolosova, Nataliya P.; Ryzhikov, Alexander B.; Taranov, Oleg S.; Omigov, Vladimir V.; Gavrilova, Elena V.; Agafonov, Alexander P.; Maksyutov, Rinat A.; Loktev, Valery B.
- Abstract
In this study, we investigated the features of the infectious process by simulating co-infection with SARS-CoV-2 and human adenovirus type 5 (HAdV-5) or influenza A virus (IAV) in vitro and in vivo. The determination of infectious activity of viruses and digital PCR demonstrated that during simultaneous and sequential HAdV-5 followed by SARS-CoV-2 infection in vitro and in vivo, the HAdV-5 infection does not interfere with replication of SARS-CoV-2. The hamsters co-infected and mono-infected with SARS-CoV-2 exhibited nearly identical viral titers and viral loads of SARS-CoV-2 in the lungs. The hamsters and ferrets co-infected by SARS-CoV-2- and IAV demonstrated more pronounced clinical manifestations than mono-infected animals. Additionally, the lung histological data illustrate that HAdV-5 or IAV and SARS-CoV-2 co-infection induces more severe pathological changes in the lungs than mono-infection. The expression of several genes specific to interferon and cytokine signaling pathways in the lungs of co-infected hamsters was more upregulated compared to single infected with SARS-CoV-2 animals. Thus, co-infection with HAdV-5 or IAV and SARS-CoV-2 leads to more severe pulmonary disease in animals.
- Subjects
INFLUENZA viruses; INFLUENZA A virus; ADENOVIRUSES; SARS-CoV-2; ANIMAL diseases; ANIMAL models in research; LUNG diseases; PLANT viruses; NEUROENDOCRINE cells
- Publication
Microorganisms, 2023, Vol 11, Issue 1, p180
- ISSN
2076-2607
- Publication type
Article
- DOI
10.3390/microorganisms11010180