We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Genome Characterization of Nocturne116, Novel Lactococcus lactis -Infecting Phage Isolated from Moth.
- Authors
Zrelovs, Nikita; Dislers, Andris; Kazaks, Andris
- Abstract
While looking for novel insect-associated phages, a unique siphophage, Nocturne116, was isolated from a deceased local moth specimen along with its host, which was identified by 16S rRNA gene sequencing as a strain of Lactococcus lactis. Next-generation sequencing and the subsequent genome annotation elaborated on herein revealed that the genome of Nocturne116 is a 25,554 bp long dsDNA molecule with 10 bp long 3′ cos overhangs and a GC content of 37.99%, comprising 52 predicted open reading frames. The complete nucleotide sequence of phage Nocturne116 genome is dissimilar to any of the already sequenced phages, save for a distant link with Lactococcus phage Q54. Functions for only 15/52 of Nocturne116 gene products could be reliably predicted using contemporary comparative genomics approaches, while 22 of its gene products do not yet have any homologous entries in the public biological sequence repositories. Despite the public availability of nearly 350 elucidated Lactococcus phage complete genomes as of now, Nocturne116 firmly stands out as a sole representative of novel phage genus.
- Subjects
LACTOCOCCUS lactis; LACTOCOCCUS; BACTERIOPHAGES; GENOMES; NUCLEOTIDE sequencing; NUCLEOTIDE sequence
- Publication
Microorganisms, 2021, Vol 9, Issue 7, p1540
- ISSN
2076-2607
- Publication type
Article
- DOI
10.3390/microorganisms9071540