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- Title
Investigation of Effect of Nanosecond Pulsed Electric Field on MCF-7 Breast Cancer Cells.
- Authors
Gyanendra, Kumar; Sandeep, Shelar; Ankur, Patel; Amitava, Roy; Ramanujam, Sarathi; Rajshri, Singh; Archana, Sharma
- Abstract
Pulsed electric field therapy is a novel non-invasive approach for cancer therapy. It serves as a cell permeability enhancing agent for cancer treatment. Nanosecond, highelectrical field pulse power technology is used for delivering variable, controllable, intracellular electrical perturbations in several biological systems. Here, we investigated the effect of nanosecond (ns) electric pulse (nsEP) as a therapeutic tool for cancer. In invitro study, the breast cancer cells (MCF-7) were exposed with electric field of ~18kV/cm intensity, ~25ns duration, at 1.5Hz in a 2mm electroporation cuvette. Post exposure, observation shows a significant reduction in cell viability. It was evident that after treatment the viability of MCF-7 cancer cells at 630 pulses are remains ~38% only. The optical microscopic analysis of MCF-7 cells shows cell morphology changes after electrical pulse exposure. Moreover, we have also investigated a comparative study of the effect nano-second electrical pulses on MCF-7 cells and Chinese Hamster Ovary (CHO) cell line. The comparative study, demonstrated that the effect of nsEPF on MCF-7 is more destructive than on CHO cell line. The obtained results support that the pulse electrical field of nanosecond (ns) duration therapy would be a potential solution for cancer treatment.
- Subjects
CHO cell; ELECTRIC fields; CANCER cells; CELL permeability; CELL morphology
- Publication
Journal of Drug Delivery & Therapeutics, 2021, Vol 11, Issue 3, p43
- ISSN
2250-1177
- Publication type
Article
- DOI
10.22270/jddt.v11i3.4827