We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Carbon nanotubes degraded by neutrophil myeloperoxidase induce less pulmonary inflammation.
- Authors
Kagan, Valerian E.; Konduru, Nagarjun V.; Weihong Feng; Allen, Brett L.; Conroy, Jennifer; Volkov, Yuri; Vlasova, Irina I.; Belikova, Natalia A.; Yanamala, Naveena; Kapralov, Alexander; Tyurina, Yulia Y.; Jingwen Shi; Kisin, Elena R.; Murray, Ashley R.; Franks, Jonathan; Stolz, Donna; Pingping Gou; Klein-Seetharaman, Judith; Fadeel, Bengt; Star, Alexander
- Abstract
We have shown previously that single-walled carbon nanotubes can be catalytically biodegraded over several weeks by the plant-derived enzyme, horseradish peroxidase. However, whether peroxidase intermediates generated inside human cells or biofluids are involved in the biodegradation of carbon nanotubes has not been explored. Here, we show that hypochlorite and reactive radical intermediates of the human neutrophil enzyme myeloperoxidase catalyse the biodegradation of single-walled carbon nanotubes in vitro, in neutrophils and to a lesser degree in macrophages. Molecular modelling suggests that interactions of basic amino acids of the enzyme with the carboxyls on the carbon nanotubes position the nanotubes near the catalytic site. Importantly, the biodegraded nanotubes do not generate an inflammatory response when aspirated into the lungs of mice. Our findings suggest that the extent to which carbon nanotubes are biodegraded may be a major determinant of the scale and severity of the associated inflammatory responses in exposed individuals.
- Subjects
HORSERADISH; BIODEGRADATION; CARBON nanotubes; ENZYMES; NEUTROPHILS; AMINO acids
- Publication
Nature Nanotechnology, 2010, Vol 5, Issue 5, p354
- ISSN
1748-3387
- Publication type
Article
- DOI
10.1038/nnano.2010.44