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- Title
Alterations in Nitric Oxide Synthase Isoforms in Acute Lower Limb Ischemia and Reperfusion.
- Authors
Tsui, Janice C. S.; Baker, Daryll M.; Shaw, Sidney G.; Dashwood, Michael R.
- Abstract
Atterations in nitric oxide synthase (NOS) are implicated in ischemia and ischemia-reperfusion injury. Changes in the 3 NOS isoforms in human skeletal muscle subjected to acute ischemia and reperfusion were studied. Muscle biopsies were taken from patients undergoing total knee replacement. Distribution of the specific NOS isoforms within muscle sections was studied using immunohistochemistry. NOS mRNA levels were measured using real-time reverse transcription-polymerase chain reaction and protein levels studied using Western blotting. NOS activity was also assessed using the citrulline assay. All 3 NOS isoforms were found in muscle sections associated with muscle fibers and microvessels. In muscle subjected to acute ischemia and reper- fusion, NOS I/neuronat NOS mRNA and protein were elevated during reperfusion. NOS Ill/endothelial NOS was also upregulated at the protein level during reperfusion. No changes in NOS Il/inducible NOS expression or NOS activity occurred. In conclusion, alterations in NOS I and Ill (neuronal NOS and endothelial NOS) at different levels occurred after acute ischemia and reperfusion in human skeletal muscle; however, this did not result in increased NOS activity. In the development of therapeutic agents based on manipulation of the NO pathway, targeting the appropriate NOS isoenzymes may be important.
- Subjects
NITRIC oxide; ISCHEMIA; REPERFUSION injury; IMMUNOHISTOCHEMISTRY; MESSENGER RNA; POLYMERASE chain reaction
- Publication
Angiology, 2007, Vol 58, Issue 5, p586
- ISSN
0003-3197
- Publication type
Article
- DOI
10.1177/0003319707305466