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- Title
Enhanced Y1H assays for Arabidopsis.
- Authors
Gaudinier, Allison; Zhang, Lifang; Reece-Hoyes, John S; Taylor-Teeples, Mallorie; Pu, Li; Liu, Zhijie; Breton, Ghislain; Pruneda-Paz, Jose L; Kim, Dahae; Kay, Steve A; Walhout, Albertha J M; Ware, Doreen; Brady, Siobhan M
- Abstract
We present an Arabidopsis thaliana full-length transcription factor resource of 92% of root stele-expressed transcription factors and 74.5% of root-expressed transcription factors. We demonstrate its use with enhanced yeast one-hybrid (eY1H) screening for rapid, systematic mapping of plant transcription factor-promoter interactions. We identified 158 interactions with 13 stele-expressed promoters, many of which occur physically or are regulatory in planta.
- Subjects
ARABIDOPSIS; YEAST; GENETIC regulation; PLANT cells &; tissues; TRANSCRIPTION factors
- Publication
Nature Methods, 2011, Vol 8, Issue 12, p1053
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.1750