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- Title
Quantitative analysis of fitness and genetic interactions in yeast on a genome scale.
- Authors
Baryshnikova, Anastasia; Costanzo, Michael; Yungil Kim; Huiming Ding; Judice Koh; Kiana Toufighi; Ji-Young Youn; Jiongwen Ou; San Luis, Bryan-Joseph; Bandyopadhyay, Sunayan; Hibbs, Matthew; Hess, David; Gingras, Anne-Claude; Bader, Gary D.; Troyanskaya, Olga G.; Brown, Grant W.; Andrews, Brenda; Boone, Charles; Myers, Chad L.
- Abstract
Global quantitative analysis of genetic interactions is a powerful approach for deciphering the roles of genes and mapping functional relationships among pathways. Using colony size as a proxy for fitness, we developed a method for measuring fitness-based genetic interactions from high-density arrays of yeast double mutants generated by synthetic genetic array (SGA) analysis. We identified several experimental sources of systematic variation and developed normalization strategies to obtain accurate single- and double-mutant fitness measurements, which rival the accuracy of other high-resolution studies. We applied the SGA score to examine the relationship between physical and genetic interaction networks, and we found that positive genetic interactions connect across functionally distinct protein complexes revealing a network of genetic suppression among loss-of-function alleles.
- Subjects
QUANTITATIVE chemical analysis; GENOMES; GENETICS; YEAST; PROTEINS
- Publication
Nature Methods, 2010, Vol 7, Issue 12, p1017
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.1534