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- Title
B cell-activating factor modulates the factor VIII immune response in hemophilia A.
- Authors
Doshi, Bhavya S.; Rana, Jyoti; Castaman, Giancarlo; Shaheen, Mostafa A.; Kaczmarek, Radoslaw; Butterfield, John S. S.; Meeks, Shannon L.; Leissinger, Cindy; Biswas, Moanaro; Arruda, Valder R.; Butterfield, John Ss
- Abstract
Inhibitors of factor VIII (FVIII) remain the most challenging complication of FVIII protein replacement therapy in hemophilia A (HA). Understanding the mechanisms that guide FVIII-specific B cell development could help identify therapeutic targets. The B cell-activating factor (BAFF) cytokine family is a key regulator of B cell differentiation in normal homeostasis and immune disorders. Thus, we used patient samples and mouse models to investigate the potential role of BAFF in modulating FVIII inhibitors. BAFF levels were elevated in pediatric and adult HA inhibitor patients and decreased to levels similar to those of noninhibitor controls after successful immune tolerance induction (ITI). Moreover, elevations in BAFF levels were seen in patients who failed to achieve FVIII tolerance with anti-CD20 antibody-mediated B cell depletion. In naive HA mice, prophylactic anti-BAFF antibody therapy prior to FVIII immunization prevented inhibitor formation and this tolerance was maintained despite FVIII exposure after immune reconstitution. In preimmunized HA mice, combination therapy with anti-CD20 and anti-BAFF antibodies dramatically reduced FVIII inhibitors via inhibition of FVIII-specific plasma cells. Our data suggest that BAFF may regulate the generation and maintenance of FVIII inhibitors and/or anti-FVIII B cells. Finally, anti-CD20/anti-BAFF combination therapy may be clinically useful for ITI.
- Subjects
BLOOD coagulation factor VIII antibodies; B cell differentiation; IMMUNE response; TALL-1 (Protein); PLASMA cells; B cells; HEMOPHILIA; IMMUNOLOGICAL tolerance; RESEARCH; IMMUNOGLOBULINS; ANIMAL experimentation; RESEARCH methodology; MEDICAL cooperation; EVALUATION research; COMPARATIVE studies; TUMOR necrosis factors; BLOOD coagulation factors; EPITHELIAL cells; MICE; CHEMICAL inhibitors
- Publication
Journal of Clinical Investigation, 2021, Vol 131, Issue 8, p1
- ISSN
0021-9738
- Publication type
journal article
- DOI
10.1172/JCI142906