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- Title
Granulosa cell tumor of the ovary. Immunohistochemical evidence of low proliferative activity and virtual absence of mutation of the p53 tumor-suppressor gene.
- Authors
Horny, H P; Marx, L; Kröber, S; Lüttges, J; Kaiserling, E; Dietl, J
- Abstract
<bold>Background and Methods: </bold>Because the use of immunohistochemistry in the diagnosis of granulosa cell tumor (GCT) has not been fully explored, routinely processed (formalin-fixed, paraffin-embedded) tissue from 11 GCT, adult type, was investigated immunohistochemically (ABC method) with a broad spectrum of antibodies against various markers, including p53 and Ki-67. All of the tumors exhibited typical morphology, were limited to the ovary (stage I), and 7 cases followed a benign clinical course.<bold>Results: </bold>All the tumors exhibited strong expression of vimentin, but most other antigens (including smooth muscle actin) were expressed infrequently by a minority of tumor cells or not at all. Tumor cells in 9 GCT expressed inhibin A. All the tumors exhibited very low proliferative activity, fewer than 10% of the tumor cell nuclei being stained by the antibody MIB-1 (Ki-67 antigen). The antibody D07 revealed marked overexpression of p53 protein in only one tumor. Clinical outcome was not found to be related to immunophenotypic differences.<bold>Conclusions: </bold>The diagnosis of GCT should be based primarily on the typical morphology revealed by conventional stains, but additional immunohistochemical staining with a small panel of selected antibodies (for example, against keratin, vimentin, and inhibin A) may be helpful in a few cases. The very low proliferative activity and the lack of overexpression of p53 protein are consistent with the benign clinical behavior of the majority of GCT.
- Subjects
PROTEIN analysis; GLYCOPROTEIN analysis; CELL division; CELL nuclei; CELL receptors; COMPARATIVE studies; CYTOSKELETAL proteins; IMMUNOHISTOCHEMISTRY; MACROPHAGES; RESEARCH methodology; MEDICAL cooperation; MUSCLE proteins; GENETIC mutation; ONCOGENES; OVARIAN tumors; RESEARCH; SMOOTH muscle; T cells; TUMOR markers; EVALUATION research; GRANULOSA cell tumors
- Publication
Gynecologic & Obstetric Investigation, 1999, Vol 47, Issue 2, p133
- ISSN
0378-7346
- Publication type
journal article