We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
The forced activation of asexual conidiation in Aspergillus niger simplifies bioproduction.
- Authors
Xingyu Wu; Tingting Zhang; Ke Zhang; Rui Zhang; Man Shi; Chenlei Gu; Tianqiong Shi; Ling Lu; Feng Xue; Qing Xu; Chi Zhang
- Abstract
Aspergillus niger is an efficient cell factory for organic acids production, particularly L-malic acid, through genetic manipulation. However, the traditional method of collecting A. niger spores for inoculation is labor-intensive and resource-consuming. In our study, we used the CRISPR-Cas9 system to replace the promoter of brlA, a key gene in Aspergillus conidiation, with a xylose-inducible promoter xylP in L-malic acid-producing A. niger strain RG0095, generating strain brlAxylP. When induced with xylose in submerged liquid culture, brlAxylP exhibited significant upregulation of conidiation-related genes. This induction allowed us to easily collect an abundance of brlAxylP spores (>7.1 × 106/mL) in liquid xylose medium. Significantly, the submerged conidiation approach preserves the substantial potential of A. niger as a foundational cellular platform for the biosynthesis of organic acids, including but not limited to L-malic acid. In summary, our study offers a simplified submerged conidiation strategy to streamline the preparation stage and reduce labor and material costs for industrial organic acid production using Aspergillus species.
- Subjects
ASPERGILLUS niger; ORGANIC acids; CRISPRS; CONIDIATION; MALIC acid; BIOSYNTHESIS
- Publication
Synthetic & Systems Biotechnology, 2024, Vol 9, Issue 2, p277
- ISSN
2097-1206
- Publication type
Article
- DOI
10.1016/j.synbio.2024.02.007