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- Title
Reassessing endothelial-to-mesenchymal transition in mouse bone marrow: insights from lineage tracing models.
- Authors
Cao, Jia; Jin, Ling; Yan, Zi-Qi; Wang, Xiao-Kai; Li, You-You; Wang, Zun; Liu, Yi-Wei; Li, Hong-Ming; Guan, Zhe; He, Ze-Hui; Gong, Jiang-Shan; Liu, Jiang-Hua; Yin, Hao; Tan, Yi-Juan; Hong, Chun-Gu; Feng, Shi-Kai; Zhang, Yan; Wang, Yi-Yi; Qi, Lu-Yue; Chen, Chun-Yuan
- Abstract
Endothelial cells (ECs) and bone marrow stromal cells (BMSCs) play crucial roles in supporting hematopoiesis and hematopoietic regeneration. However, whether ECs are a source of BMSCs remains unclear. Here, we evaluate the contribution of endothelial-to-mesenchymal transition to BMSC generation in postnatal mice. Single-cell RNA sequencing identifies ECs expressing BMSC markers Prrx1 and Lepr; however, this could not be validated using Prrx1-Cre and Lepr-Cre transgenic mice. Additionally, only a minority of BMSCs are marked by EC lineage tracing models using Cdh5-rtTA-tetO-Cre or Tek-CreERT2. Moreover, Cdh5+ BMSCs and Tek+ BMSCs show distinct spatial distributions and characteristic mesenchymal markers, suggestive of their origination from different progenitors rather than CDH5+ TEK+ ECs. Furthermore, myeloablation induced by 5-fluorouracil treatment does not increase Cdh5+ BMSCs. Our findings indicate that ECs hardly convert to BMSCs during homeostasis and myeloablation-induced hematopoietic regeneration, highlighting the importance of using appropriate genetic models and conducting careful data interpretation in studies concerning endothelial-to-mesenchymal transition. Endothelial cells (ECs) and bone marrow stromal cells (BMSCs) support hematopoiesis and hematopoietic regeneration. Whether ECs are a source of BMSCs remains unclear. Here, using lineage tracing models the authors show that ECs are not a source of BMSCs during homeostasis and regeneration.
- Subjects
MESENCHYMAL stem cells; HOMEOSTASIS; BONE marrow; TRANSGENIC mice; GENETIC models; ENDOTHELIAL cells
- Publication
Nature Communications, 2023, Vol 14, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-023-44312-w