We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Molecular detection of Salmonella species in bovine fecal samples.
- Authors
Bordonaro, Rebecca; McDonough, Patrick L.; Chang, Yung-fu; Mohammed, Hussni O.
- Abstract
A study was performed to assess the validity of the BAX automated polymerase chain reaction system (DuPont Nutrition & Health, Wilmington, Delaware) to detect the shedding of Salmonella species in bovine fecal samples. A total of 133 bovine fecal samples that were submitted to the Cornell University Animal Health Diagnostic Laboratory for Salmonella culture were also tested in the BAX system with a modified version of the manufacturer’s enrichment protocol. Using culture as the gold standard test, the BAX system was found to have a sensitivity of 85.7% and a specificity of 90.5%. There was excellent agreement (κ = 0.71, standard error = 0.072) and no significant differences between the 2 methods (McNemar χ2 = 0.180).
- Subjects
MOLECULAR diagnosis; ANIMAL dropping analysis; SALMONELLA infections in cattle; VETERINARY diagnosis; POLYMERASE chain reaction
- Publication
Journal of Veterinary Diagnostic Investigation, 2013, Vol 25, Issue 6, p756
- ISSN
1040-6387
- Publication type
Article
- DOI
10.1177/1040638713508123